PCR Supplies





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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - EGFR 6225
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.


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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - KIT 1314
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.


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Applied Biosystems™ TrueMark™ Amplification Control I (1 x 105 copies/μL)
The TrueMark Amplification Control I for TrueMark plates contains a linearized multi-target plasmid pool with microbial target sequences used on TrueMark infectious disease research panels.


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Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.


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Shipping Condition | Dry Ice |
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InhibitorToleranceSimple | High |
Concentration | 4X |
For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
PCR Method | 1-step RT-qPCR |
Multiplex Capability | Up to 4-plex |
Detection Method | Primer-probe |
Thermostability | Not Applicable |
Passive Reference Dye | None |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | RNA, DNA |
Reaction Speed | Fast or Standard |
Target Specificity | RNA only, Both RNA and DNA |
Hot Start | Built-In Hot Start |
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.


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Content And Storage | Store at 2°C to 8°C in the dark. |
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Form | Liquid |
InhibitorToleranceSimple | High |
Concentration | 2X |
For Use With (Application) | Gene Expression,Microbial Detection,DNA Quantitation,Presence/Absence,miRNA analysis |
PCR Method | qPCR |
Multiplex Capability | Duplex |
Detection Method | Primer-probe |
Includes | dUTP, UNG/UDG |
Thermostability | 48 hr., Assembled Reaction |
Polymerase | Fast DNA Polymerase |
Sensitivity | Down to 10 copies |
Format | Bottle |
Product Type | qPCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | QuantStudio™ Dx, 7500 Fast Dx, QuantStudio™ Absolute Q Digital PCR System |
Sample Type | cDNA, DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Hot Start | Built-In Hot Start |
Product Line | TaqPath™ |
Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit, 384-well
TaqMan™ hPSC Scorecard™ Kit, 384-well


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Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.


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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | M-MLV |
Green Features | Fewer resources used and less waste, Less hazardous |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | DNA Polymerase |
Format | Tube |
Product Type | Gene Expression Cells-to-C Kit |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell |
Reaction Speed | Standard |
Product Line | Ambion™, Cells-to-CT™, TaqMan™ |
Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.


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Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.


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Shipping Condition | Dry Ice |
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Polymerase | Taq DNA Polymerase |
Product Type | One-Step qRT-PCR Kit |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | Platinum™, SuperScript™ |
Detection Method | Primer-probe |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).


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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Format | Super Mix |
Reverse Transcriptase | SuperScript III |
Reaction Format | Master Mix |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.


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Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript II |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.


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GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Quantity | Each |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR
The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.


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Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.


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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Kit |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.


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Polymerase | Taq DNA Polymerase |
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Format | Tube |
Product Type | qRT-PCR Kit |
For Use With (Equipment) | 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ |
Sample Type | RNA |
Reaction Speed | Fast or Standard |
PCR Method | 1-step RT-qPCR |
Product Line | SuperScript™ |
Detection Method | Primer-probe |